› 5avn

X-ray diffraction
1.03Å resolution

The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel

Released:
DOI: 10.2210/pdb5avn/pdb
PDB entry 5avn coloured by chain, front view.
PDB entry 5avn coloured by chain, side view.
PDB entry 5avn coloured by chain, top view.
Source organism: Streptomyces rubiginosus
Primary publication:
Growth of protein crystals in hydrogels prevents osmotic shock.
Sugiyama S, Maruyama M, Sazaki G, Hirose M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Matsumura H
J Am Chem Soc 134 5786-9 (2012)
PMID: 22435400

Function and Biology Details

Reaction catalysed: 
Alpha-D-xylopyranose = alpha-D-xylufuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-150202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 43.15 KDa
Source organism: Streptomyces rubiginosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24300 (Residues: 2-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand CA 2 x CA
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21212
Unit cell:
a: 94.082Å b: 98.285Å c: 85.777Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 0.127 0.15
Expression system: Escherichia coli

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Citations

3 review citations

An overview of biological macromolecule crystallization.
Russo Krauss et al. (2013)
2 more