› 5ax1

X-ray diffraction
1.8Å resolution

Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.80 angstrom

Released:
DOI: 10.2210/pdb5ax1/pdb
PDB entry 5ax1 coloured by chain, front view.
PDB entry 5ax1 coloured by chain, side view.
PDB entry 5ax1 coloured by chain, top view.
Source organism: Acetabularia acetabulum
Primary publication:
Structural basis for the slow photocycle and late proton release in Acetabularia rhodopsin I from the marine plant Acetabularia acetabulum.
Furuse M, Tamogami J, Hosaka T, Kikukawa T, Shinya N, Hato M, Ohsawa N, Kim SY, Jung KH, Demura M, Miyauchi S, Kamo N, Shimono K, Kimura-Someya T, Yokoyama S, Shirouzu M
Acta Crystallogr D Biol Crystallogr 71 2203-16 (2015)
PMID: 26527138

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin I Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 26.48 KDa
Source organism: Acetabularia acetabulum
Expression system: Escherichia coli
UniProt:
  • Canonical: G3CEP6 (Residues: 1-237; Coverage: 96%)
Gene name: aopI
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

6 bound ligands:
Ligand RET 1 x RET
Ligand OLB 2 x OLB
Ligand C14 1 x C14
Ligand D10 2 x D10
Ligand R16 1 x R16
Ligand D12 2 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 75.99Å b: 101.63Å c: 43.76Å
α: 90° β: 119.17° γ: 90°
R-values:
R R work R free
0.176 0.175 0.204
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.
Inoue K. (2021)
1 more