› 5ayy

X-ray diffraction
3.09Å resolution

CRYSTAL STRUCTURE OF HUMAN QUINOLINATE PHOSPHORIBOSYLTRANSFERASE IN COMPLEX WITH THE REACTANT QUINOLINATE

Released:
DOI: 10.2210/pdb5ayy/pdb
PDB entry 5ayy coloured by chain, front view.
PDB entry 5ayy coloured by chain, side view.
PDB entry 5ayy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into the Quaternary Catalytic Mechanism of Hexameric Human Quinolinate Phosphoribosyltransferase, a Key Enzyme in de novo NAD Biosynthesis.
Youn HS, Kim TG, Kim MK, Kang GB, Kang JY, Lee JG, An JY, Park KR, Lee Y, Im YJ, Lee JH, Eom SH
Sci Rep 6 19681 (2016)
PMID: 26805589

Function and Biology Details

Reaction catalysed: 
Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 305 amino acids
Theoretical weight: 31.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15274 (Residues: 1-297; Coverage: 100%)
Gene name: QPRT
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NTM 9 x NTM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3221
Unit cell:
a: 174.245Å b: 174.245Å c: 211.684Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.185 0.239
Expression system: Escherichia coli

Quick links

Citations

5 review citations

NAD+ metabolism: pathophysiologic mechanisms and therapeutic potential.
Xie et al. (2020)
4 more

2 mentions without citation

Major Developments in the Design of Inhibitors along the Kynurenine Pathway.
Jacobs et al. (2017)
1 more