› 5azp

X-ray diffraction
1.69Å resolution

Crystal structure of a membrane protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5azp/pdb
PDB entry 5azp coloured by chain, front view.
PDB entry 5azp coloured by chain, side view.
PDB entry 5azp coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structures of OprN and OprJ, outer membrane factors of multidrug tripartite efflux pumps of Pseudomonas aeruginosa.
Yonehara R, Yamashita E, Nakagawa A
Proteins 84 759-69 (2016)
PMID: 26914226

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191253 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug efflux outer membrane protein OprN Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 455 amino acids
Theoretical weight: 49.69 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I0Y7 (Residues: 26-472; Coverage: 100%)
Gene names: PA2495, oprN
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand 3PK 2 x 3PK
Ligand OYA 2 x OYA
Ligand BOG 8 x BOG
Ligand GOL 12 x GOL
Ligand FMT 18 x FMT
Ligand NA 3 x NA
Ligand ACT 9 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I4
Unit cell:
a: 257.515Å b: 257.515Å c: 81.825Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.18
Expression system: Escherichia coli

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Citations

4 review citations

Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective.
Greene et al. (2018)
3 more

3 mentions without citation

Inward-facing glycine residues create sharp turns in β-barrel membrane proteins.
Zhang et al. (2021)
2 more