› 5azs

X-ray diffraction
3.1Å resolution

Crystal structure of a membrane protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5azs/pdb
PDB entry 5azs coloured by chain, front view.
PDB entry 5azs coloured by chain, side view.
PDB entry 5azs coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structures of OprN and OprJ, outer membrane factors of multidrug tripartite efflux pumps of Pseudomonas aeruginosa.
Yonehara R, Yamashita E, Nakagawa A
Proteins 84 759-69 (2016)
PMID: 26914226

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein OprJ Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 468 amino acids
Theoretical weight: 51.08 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51397 (Residues: 20-479; Coverage: 100%)
Gene names: PA4597, oprJ
Sequence domains: Outer membrane efflux protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 86.329Å b: 92.388Å c: 252.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.269 0.307
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Antibiotic Resistance Mediated by the MacB ABC Transporter Family: A Structural and Functional Perspective.
Greene et al. (2018)
2 more

4 mentions without citation

The Treponema pallidum Outer Membrane.
Radolf et al. (2018)
3 more