› 5b0h

X-ray diffraction
1.94Å resolution

CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE CELL-DERIVED CHEMOTAXIN 2

Released:
DOI: 10.2210/pdb5b0h/pdb
PDB entry 5b0h coloured by chain, front view.
PDB entry 5b0h coloured by chain, side view.
PDB entry 5b0h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of Human Leukocyte Cell-derived Chemotaxin 2 (LECT2) Reveals a Mechanistic Basis of Functional Evolution in a Mammalian Protein with an M23 Metalloendopeptidase Fold.
Zheng H, Miyakawa T, Sawano Y, Asano A, Okumura A, Yamagoe S, Tanokura M
J Biol Chem 291 17133-42 (2016)
PMID: 27334921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leukocyte cell-derived chemotaxin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 14.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14960 (Residues: 19-151; Coverage: 100%)
Gene name: LECT2
Sequence domains: Peptidase family M23
Structure domains: Glucose Permease (Domain IIA)

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 59.415Å b: 63.514Å c: 63.976Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.223
Expression system: Escherichia coli

Quick links

Citations

5 review citations

LECT2: A pleiotropic and promising hepatokine, from bench to bedside.
Xie et al. (2022)
4 more

3 mentions without citation

Crystal Structure of Human Leukocyte Cell-derived Chemotaxin 2 (LECT2) Reveals a Mechanistic Basis of Functional Evolution in a Mammalian Protein with an M23 Metalloendopeptidase Fold.
Zheng et al. (2016)
2 more