› 5b2g

X-ray diffraction
3.5Å resolution

Crystal structure of human claudin-4 in complex with C-terminal fragment of Clostridium perfringens enterotoxin

Released:
DOI: 10.2210/pdb5b2g/pdb
PDB entry 5b2g coloured by chain, front view.
PDB entry 5b2g coloured by chain, side view.
PDB entry 5b2g coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin.
Shinoda T, Shinya N, Ito K, Ohsawa N, Terada T, Hirata K, Kawano Y, Yamamoto M, Kimura-Someya T, Yokoyama S, Shirouzu M
Sci Rep 6 33632 (2016)
PMID: 27647526

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126979 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Claudin-4; Endolysin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 364 amino acids
Theoretical weight: 39.41 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
  • Canonical: O14493 (Residues: 1-183; Coverage: 88%)
Gene names: CLDN4, CPER, CPETR1, E, WBSCR8
Sequence domains:
Heat-labile enterotoxin B chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 142 amino acids
Theoretical weight: 15.88 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01558 (Residues: 187-319; Coverage: 42%)
Gene name: cpe
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P43
Unit cell:
a: 105.92Å b: 105.92Å c: 244.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.289 0.288 0.309
Expression system: Escherichia coli

Quick links

Citations

12 review citations

An update on the human and animal enteric pathogen Clostridium perfringens.
Kiu et al. (2018)
11 more

14 mentions without citation

Structural Features of Tight-Junction Proteins.
Heinemann et al. (2019)
13 more