› 5b4a

X-ray diffraction
1.72Å resolution

Crystal structure of LpxH with lipid X in spacegroup P21

Released:
DOI: 10.2210/pdb5b4a/pdb
PDB entry 5b4a coloured by chain, front view.
PDB entry 5b4a coloured by chain, side view.
PDB entry 5b4a coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structures of the UDP-diacylglucosamine pyrophosphohydrase LpxH from Pseudomonas aeruginosa.
Okada C, Wakabayashi H, Kobayashi M, Shinoda A, Tanaka I, Yao M
Sci Rep 6 32822 (2016)
PMID: 27609419

Function and Biology Details

Reaction catalysed: 
A UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + H(2)O = a lipid X + UMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2,3-diacylglucosamine hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.67 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I2V0 (Residues: 1-240; Coverage: 100%)
Gene names: PA1792, lpxH
Sequence domains: Calcineurin-like phosphoesterase

Ligands and Environments

2 bound ligands:
Ligand LP5 2 x LP5
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 49.016Å b: 108.805Å c: 51.685Å
α: 90° β: 92.26° γ: 90°
R-values:
R R work R free
0.161 0.16 0.188
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, inhibition, and regulation of essential lipid A enzymes.
Zhou et al. (2017)
3 more

3 mentions without citation

Crystal structures of the UDP-diacylglucosamine pyrophosphohydrase LpxH from Pseudomonas aeruginosa.
Okada et al. (2016)
2 more