› 5b58

X-ray diffraction
3.21Å resolution

Inward-facing conformation of ABC heme importer BhuUV in complex with periplasmic heme binding protein BhuT from Burkholderia cenocepacia

Released:
DOI: 10.2210/pdb5b58/pdb
PDB entry 5b58 coloured by chain, front view.
PDB entry 5b58 coloured by chain, side view.
PDB entry 5b58 coloured by chain, top view.
Source organism: Burkholderia cenocepacia J2315
Primary publication:
Crystal structure of bacterial haem importer complex in the inward-facing conformation.
Naoe Y, Nakamura N, Doi A, Sawabe M, Nakamura H, Shiro Y, Sugimoto H
Nat Commun 7 13411 (2016)
PMID: 27830695

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-110054 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemin ABC transport system, membrane protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 38.8 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB4 (Residues: 1-362; Coverage: 100%)
Gene names: BCAM2629, hmuU
Sequence domains: FecCD transport family
Structure domains: ABC transporter involved in vitamin B12 uptake, BtuC
ABC transporter domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 273 amino acids
Theoretical weight: 29.23 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB5 (Residues: 1-273; Coverage: 100%)
Gene names: BCAM2630, hmuV
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Fe/B12 periplasmic-binding domain-containing protein Chain: T
Molecule details ›
Chain: T
Length: 271 amino acids
Theoretical weight: 28.02 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EKB3 (Residues: 40-305; Coverage: 87%)
Gene names: BCAM2628, hmuT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 69.016Å b: 99.751Å c: 253.496Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.269 0.31
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Extracellular Heme Uptake and the Challenge of Bacterial Cell Membranes.
Huang et al. (2017)
3 more

7 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
6 more