› 5b7c

X-ray diffraction
2.35Å resolution

Crystal structure of octopus S-crystallin Q108F mutant in complex with glutathione

Released:
DOI: 10.2210/pdb5b7c/pdb
PDB entry 5b7c coloured by chain, front view.
PDB entry 5b7c coloured by chain, side view.
PDB entry 5b7c coloured by chain, top view.
Source organism: Octopus vulgaris
Primary publication:
Structure of a Highly Active Cephalopod S-crystallin Mutant: New Molecular Evidence for Evolution from an Active Enzyme into Lens-Refractive Protein.
Tan WH, Cheng SC, Liu YT, Wu CG, Lin MH, Chen CC, Lin CH, Chou CY
Sci Rep 6 31176 (2016)
PMID: 27499004

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-crystallin OctvuS4 Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 28.03 KDa
Source organism: Octopus vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1C7D1H4 (Residues: 1-215; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GSH 1 x GSH
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P6422
Unit cell:
a: 114.322Å b: 114.322Å c: 63.927Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.25
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription.
Belashov et al. (2018)
5 more

1 mention without citation

Structure of a Highly Active Cephalopod S-crystallin Mutant: New Molecular Evidence for Evolution from an Active Enzyme into Lens-Refractive Protein.
Tan et al. (2016)