› 5b7j

Solution NMR

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118425 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Switch-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 13.25 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: P40847 (Residues: 25-135; Coverage: 44%)
Gene names: SPCC1672.02c, sap1
Sequence domains: Switch activating protein 1, N-terminal
DNA (5'-D(*CP*AP*AP*AP*AP*CP*AP*AP*TP*AP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 12 nucleotides
Theoretical weight: 3.64 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided
DNA (5'-D(*AP*AP*TP*AP*TP*TP*GP*TP*TP*TP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 48%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Approaching Protein Barriers: Emerging Mechanisms of Replication Pausing in Eukaryotes.
Shyian et al. (2021)
3 other articles