› 5b83

X-ray diffraction
2.69Å resolution

Crystal structure of Optineurin UBAN in complex with linear ubiquitin

Released:
DOI: 10.2210/pdb5b83/pdb
PDB entry 5b83 coloured by chain, front view.
PDB entry 5b83 coloured by chain, side view.
PDB entry 5b83 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis.
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F
Nat Commun 7 12547 (2016)
PMID: 27552911

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143394 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chains: A, D
Molecule details ›
Chains: A, D
Length: 304 amino acids
Theoretical weight: 34.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 381-684; Coverage: 44%)
Gene name: UBC
Sequence domains: Ubiquitin family
Optineurin Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 102 amino acids
Theoretical weight: 11.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CV9 (Residues: 416-510; Coverage: 17%)
Gene names: FIP2, GLC1E, HIP7, HYPL, NRP, OPTN
Sequence domains: Leucine zipper of domain CC2 of NEMO, NF-kappa-B essential modulator

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 71.327Å b: 82.038Å c: 244.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.254
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Necroptosis and RIPK1-mediated neuroinflammation in CNS diseases.
Yuan et al. (2019)
32 more

2 mentions without citation

The role of protein complexes in human genetic disease.
Bergendahl et al. (2019)
1 more