› 5bkm

X-ray diffraction
2.7Å resolution

Crystal Structure of Hip1 (Rv2224c) mutant - S228DHA (dehydroalanine)

Released:
DOI: 10.2210/pdb5bkm/pdb
PDB entry 5bkm coloured by chain, front view.
PDB entry 5bkm coloured by chain, side view.
PDB entry 5bkm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Naffin-Olivos JL, Daab A, Goldfarb NE, Doran MH, Baikovitz J, Liu D, Sun S, White A, Dunn BM, Rengarajan J, Petsko GA, Ringe D

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease Hip1 Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 52.97 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WHR3 (Residues: 14-14, 35-520; Coverage: 93%)
Gene names: MTCY427.05c, Rv2224c, caeA, hip1
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand DHA 1 x DHA

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 104.444Å b: 104.444Å c: 127.477Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.242
Expression system: Escherichia coli BL21(DE3)

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