› 5bng

X-ray diffraction
3.5Å resolution

monomer of TALE type homeobox transcription factor MEIS1 complexes with specific DNA

Released:
DOI: 10.2210/pdb5bng/pdb
PDB entry 5bng coloured by chain, front view.
PDB entry 5bng coloured by chain, side view.
PDB entry 5bng coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA-dependent formation of transcription factor pairs alters their binding specificity.
Jolma A, Yin Y, Nitta KR, Dave K, Popov A, Taipale M, Enge M, Kivioja T, Morgunova E, Taipale J
Nature 527 384-8 (2015)
PMID: 26550823

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-218986 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Homeobox protein Meis2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 60 amino acids
Theoretical weight: 7.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14770 (Residues: 283-342; Coverage: 13%)
Gene names: MEIS2, MRG1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
DNA (5'-D(P*TP*TP*AP*GP*CP*TP*GP*TP*CP*A)-3') Chain: L
Molecule details ›
Chain: L
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*GP*AP*CP*AP*GP*CP*TP*AP*A-3') Chain: M
Molecule details ›
Chain: M
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*AP*AP*TP*TP*AP*GP*CP*TP*GP*TP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*GP*AP*CP*AP*GP*CP*TP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 45.9Å b: 59.94Å c: 107.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.345 0.343 0.388
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

52 review citations

Ever-Changing Landscapes: Transcriptional Enhancers in Development and Evolution.
Long et al. (2016)
51 more

1 mention without citation

Development of Small Molecule MEIS Inhibitors that modulate HSC activity.
Turan et al. (2020)