› 5bpu

X-ray diffraction
2.4Å resolution

Crystal structure of Norrin, a Wnt signalling activator, Crystal Form I

Released:
DOI: 10.2210/pdb5bpu/pdb
PDB entry 5bpu coloured by chain, front view.
PDB entry 5bpu coloured by chain, side view.
PDB entry 5bpu coloured by chain, top view.
Source organisms:
Primary publication:
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Chang TH, Hsieh FL, Zebisch M, Harlos K, Elegheert J, Jones EY
Elife 4 (2015)
PMID: 26158506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165459 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Norrin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 122 amino acids
Theoretical weight: 13.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00604 (Residues: 25-133; Coverage: 100%)
Gene names: EVR2, NDP
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
(GGL)EEEEEE Chain: H
Molecule details ›
Chain: H
Length: 7 amino acids
Theoretical weight: 922 Da
Source organism: synthetic construct
Expression system: Not provided
(GGL)EEE Chain: I
Molecule details ›
Chain: I
Length: 4 amino acids
Theoretical weight: 534 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GGL 2 x GGL

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 46.37Å b: 79.1Å c: 234.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.263
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

18 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
17 more

4 mentions without citation

Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Chang et al. (2015)
3 more