› 5bq8

X-ray diffraction
2Å resolution

Crystal structure of Norrin, a Wnt signalling activator, Crystal Form II

Released:
DOI: 10.2210/pdb5bq8/pdb
PDB entry 5bq8 coloured by chain, front view.
PDB entry 5bq8 coloured by chain, side view.
PDB entry 5bq8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Chang TH, Hsieh FL, Zebisch M, Harlos K, Elegheert J, Jones EY
Elife 4 (2015)
PMID: 26158506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169449 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Norrin Chains: A, C
Molecule details ›
Chains: A, C
Length: 122 amino acids
Theoretical weight: 13.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00604 (Residues: 25-133; Coverage: 100%)
Gene names: EVR2, NDP
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
Norrin Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 13.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00604 (Residues: 25-133; Coverage: 100%)
Gene names: EVR2, NDP
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines
Norrin Chain: D
Molecule details ›
Chain: D
Length: 122 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00604 (Residues: 25-133; Coverage: 100%)
Gene names: EVR2, NDP
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand PEG 1 x PEG
1 modified residue:
Ligand MLY 11 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 102.667Å b: 53.059Å c: 96.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.233 0.248
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
18 more

2 mentions without citation

Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Chang et al. (2015)
1 more