› 5bqb

X-ray diffraction
2.3Å resolution

Crystal structure of Norrin, a Wnt signalling activator, Crystal Form III

Released:
DOI: 10.2210/pdb5bqb/pdb
PDB entry 5bqb coloured by chain, front view.
PDB entry 5bqb coloured by chain, side view.
PDB entry 5bqb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan.
Chang TH, Hsieh FL, Zebisch M, Harlos K, Elegheert J, Jones EY
Elife 4 (2015)
PMID: 26158506

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Norrin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 122 amino acids
Theoretical weight: 13.61 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q00604 (Residues: 25-133; Coverage: 100%)
Gene names: EVR2, NDP
Sequence domains: Cystine-knot domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

2 bound ligands:
Ligand CIT 4 x CIT
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 86.761Å b: 38.095Å c: 177.197Å
α: 90° β: 93.97° γ: 90°
R-values:
R R work R free
0.223 0.221 0.251
Expression system: Homo sapiens

Quick links

Citations

17 review citations

Wnt/β-Catenin Signaling, Disease, and Emerging Therapeutic Modalities.
Nusse et al. (2017)
16 more

3 mentions without citation

The structural biology of canonical Wnt signalling.
Agostino et al. (2020)
2 more