› 5bsz

X-ray diffraction
2Å resolution

X-ray structure of the sugar N-methyltransferase KedS8 from Streptoalloteichus sp ATCC 53650

Released:
DOI: 10.2210/pdb5bsz/pdb
PDB entry 5bsz coloured by chain, front view.
PDB entry 5bsz coloured by chain, side view.
PDB entry 5bsz coloured by chain, top view.
Source organism: Streptoalloteichus sp. ATCC 53650
Primary publication:
Molecular architecture of KedS8, a sugar N-methyltransferase from Streptoalloteichus sp. ATCC 53650.
Delvaux NA, Thoden JB, Holden HM
Protein Sci 24 1593-9 (2015)
PMID: 26177844

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125555 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 27.11 KDa
Source organism: Streptoalloteichus sp. ATCC 53650
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K4P132 (Residues: 1-248; Coverage: 100%)
Sequence domains: Methyltransferase domain

Ligands and Environments

3 bound ligands:
Ligand SAH 1 x SAH
Ligand EDO 4 x EDO
Ligand THM 1 x THM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I222
Unit cell:
a: 63.254Å b: 81.004Å c: 126.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Molecular architecture of KedS8, a sugar N-methyltransferase from Streptoalloteichus sp. ATCC 53650.
Delvaux et al. (2015)
1 more