› 5bud

X-ray diffraction
1.99Å resolution

Crystal structure of Candida albicans Rai1 in complex with pU5-Mn2+

Released:
DOI: 10.2210/pdb5bud/pdb
PDB entry 5bud coloured by chain, front view.
PDB entry 5bud coloured by chain, side view.
PDB entry 5bud coloured by chain, top view.
Source organisms:
Primary publication:
Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes.
Wang VY, Jiao X, Kiledjian M, Tong L
Nucleic Acids Res 43 6596-606 (2015)
PMID: 26101253

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176867 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Decapping nuclease RAI1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 45.88 KDa
Source organism: Candida albicans SC5314
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5AAT0 (Residues: 1-391; Coverage: 100%)
Gene names: CAALFM_C106800WA, CaO19.13610, CaO19.6230, RAI1
Sequence domains: RAI1 like PD-(D/E)XK nuclease
RNA (5'-R(P*UP*UP*UP*U)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 5 nucleotides
Theoretical weight: 1.49 KDa
Source organism: Candida albicans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 6 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 97.854Å b: 114.638Å c: 84.458Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.243
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

New insights into decapping enzymes and selective mRNA decay.
Grudzien-Nogalska et al. (2017)
4 more

1 mention without citation

Structural and biochemical studies of the distinct activity profiles of Rai1 enzymes.
Wang et al. (2015)