5bwk

X-ray diffraction
6Å resolution

6.0 A Crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
PDBe Complex ID:
PDB-CPX-171302 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATPase GET3 Chains: A, B, C, D, M, N, O, P
Molecule details ›
Chains: A, B, C, D, M, N, O, P
Length: 373 amino acids
Theoretical weight: 41.58 KDa
Source organism: Saccharomyces cerevisiae RM11-1a
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12154 (Residues: 2-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Golgi to ER traffic protein 4 Chains: E, G, I, K, Q, S, U, W
Molecule details ›
Chains: E, G, I, K, Q, S, U, W
Length: 319 amino acids
Theoretical weight: 37.25 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12125 (Residues: 11-311; Coverage: 97%)
Gene names: GET4, O3580, YOR164C
Sequence domains: Golgi to ER traffic protein 4
Ubiquitin-like protein MDY2 Chains: F, H, J, L, R, T, V, X

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 152.43Å b: 127.33Å c: 210.26Å
α: 90° β: 110.23° γ: 90°
R-values:
R R work R free
0.274 0.274 0.302
Expression system: Escherichia coli