› 5bxa

X-ray diffraction
1.9Å resolution

Structure of PslG from Pseudomonas aeruginosa in complex with mannose

Released:
DOI: 10.2210/pdb5bxa/pdb
PDB entry 5bxa coloured by chain, front view.
PDB entry 5bxa coloured by chain, side view.
PDB entry 5bxa coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Characterization of the Pseudomonas aeruginosa Glycoside Hydrolase PslG Reveals That Its Levels Are Critical for Psl Polysaccharide Biosynthesis and Biofilm Formation.
Baker P, Whitfield GB, Hill PJ, Little DJ, Pestrak MJ, Robinson H, Wozniak DJ, Howell PL
J Biol Chem 290 28374-28387 (2015)
PMID: 26424791

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PslG Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 47.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I1N2 (Residues: 31-442; Coverage: 93%)
Gene names: PA2237, pslG

Ligands and Environments

4 bound ligands:
Ligand CD 4 x CD
Ligand MAN 4 x MAN
Ligand CL 1 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P41212
Unit cell:
a: 83.655Å b: 83.655Å c: 163.366Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.187
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

What's on the Outside Matters: The Role of the Extracellular Polymeric Substance of Gram-negative Biofilms in Evading Host Immunity and as a Target for Therapeutic Intervention.
Gunn et al. (2016)
10 more

2 mentions without citation

Characterization of the Pseudomonas aeruginosa Glycoside Hydrolase PslG Reveals That Its Levels Are Critical for Psl Polysaccharide Biosynthesis and Biofilm Formation.
Baker et al. (2015)
1 more