› 5by4

X-ray diffraction
1.7Å resolution

Structure and function of the Escherichia coli Tol-Pal stator protein TolR

Released:
DOI: 10.2210/pdb5by4/pdb
PDB entry 5by4 coloured by chain, front view.
PDB entry 5by4 coloured by chain, side view.
PDB entry 5by4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and function of the Escherichia coli Tol-Pal stator protein TolR.
Wojdyla JA, Cutts E, Kaminska R, Papadakos G, Hopper JT, Stansfeld PJ, Staunton D, Robinson CV, Kleanthous C
J Biol Chem 290 26675-87 (2015)
PMID: 26354441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tol-Pal system protein TolR Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 11.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABV6 (Residues: 36-142; Coverage: 75%)
Gene names: JW0728, b0738, tolR
Sequence domains: Biopolymer transport protein ExbD/TolR

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6522
Unit cell:
a: 51.727Å b: 51.727Å c: 155.029Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.175 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Bacterial outer membrane constriction.
Egan AJF. (2018)
5 more

5 mentions without citation

The multifarious roles of Tol-Pal in Gram-negative bacteria.
Szczepaniak et al. (2020)
4 more