5bym

X-ray diffraction
2.71Å resolution

Crystal structure of the RNA-binding domain of yeast Puf5p bound to SMX2 RNA

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-153802 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Suppressor protein MPT5 Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P39016 (Residues: 201-600; Coverage: 47%)
Gene names: BIC834, HTR1, MPT5, PUF5, YGL178W
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
RNA (5'-R(*UP*GP*UP*AP*CP*UP*AP*UP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 94.215Å b: 100.792Å c: 49.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.241 0.285
Expression systems:
  • Escherichia coli
  • Not provided