› 5c1m

X-ray diffraction
2.07Å resolution

Crystal structure of active mu-opioid receptor bound to the agonist BU72

Released:
DOI: 10.2210/pdb5c1m/pdb
PDB entry 5c1m coloured by chain, front view.
PDB entry 5c1m coloured by chain, side view.
PDB entry 5c1m coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into µ-opioid receptor activation.
Huang W, Manglik A, Venkatakrishnan AJ, Laeremans T, Feinberg EN, Sanborn AL, Kato HE, Livingston KE, Thorsen TS, Kling RC, Granier S, Gmeiner P, Husbands SM, Traynor JR, Weis WI, Steyaert J, Dror RO, Kobilka BK
Nature 524 315-21 (2015)
PMID: 26245379

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229956 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mu-type opioid receptor Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.73 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P42866 (Residues: 52-347; Coverage: 74%)
Gene names: Mor, Oprm, Oprm1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
Nanobody 39 Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 13.67 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

5 bound ligands:
Ligand OLC 2 x OLC
Ligand CLR 1 x CLR
Ligand PO4 1 x PO4
Ligand P6G 2 x P6G
Ligand VF1 1 x VF1
1 modified residue:
Ligand YCM 1 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I212121
Unit cell:
a: 44.43Å b: 144Å c: 209.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.229
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

80 review citations

DREADDs for Neuroscientists.
Roth BL. (2016)
79 more

185 mentions without citation

The Molecular Basis of G Protein-Coupled Receptor Activation.
Weis et al. (2018)
184 more