› 5c32

X-ray diffraction
3.05Å resolution

Constitutively active Sin recombinase cataltyic domain - I100T

Released:
DOI: 10.2210/pdb5c32/pdb
PDB entry 5c32 coloured by chain, front view.
PDB entry 5c32 coloured by chain, side view.
PDB entry 5c32 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Snapshots of a molecular swivel in action.
Trejo CS, Rock RS, Stark WM, Boocock MR, Rice PA
Nucleic Acids Res 46 5286-5296 (2018)
PMID: 29315406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transposon Tn552 DNA-invertase bin3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 15.15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20384 (Residues: 1-128; Coverage: 63%)
Gene name: bin3
Sequence domains: Resolvase, N terminal domain
Structure domains: Resolvase, N-terminal catalytic domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 83.774Å b: 114.194Å c: 146.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.261 0.314
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination.
Mandali et al. (2021)
1 more

2 mentions without citation

Global methylation profiling of lung cancer identifies novel methylated genes.
Dai et al. (2001)
1 more