› 5c35

X-ray diffraction
2.4Å resolution

Constitutively active Sin recombinase cataltyic domain - T77II100T/Q115R

Released:
DOI: 10.2210/pdb5c35/pdb
PDB entry 5c35 coloured by chain, front view.
PDB entry 5c35 coloured by chain, side view.
PDB entry 5c35 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Snapshots of a molecular swivel in action
Trejo CS, Rock RS, Star WM, Boocock MR, Rice PA
Nucleic Acids Res. (2018)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transposon Tn552 DNA-invertase bin3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 15.19 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20384 (Residues: 1-128; Coverage: 63%)
Gene name: bin3
Sequence domains: Resolvase, N terminal domain
Structure domains: Resolvase, N-terminal catalytic domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 73.842Å b: 73.842Å c: 180.483Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.318 0.316 0.335
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination.
Mandali et al. (2021)
1 more

1 mention without citation

Snapshots of a molecular swivel in action.
Trejo et al. (2018)