› 5c4l

X-ray diffraction
2.35Å resolution

Conformational alternate of sisomicin in complex with APH(2")-IVa

Released:
DOI: 10.2210/pdb5c4l/pdb
PDB entry 5c4l coloured by chain, front view.
PDB entry 5c4l coloured by chain, side view.
PDB entry 5c4l coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Aminoglycoside binding and catalysis specificity of aminoglycoside 2″-phosphotransferase IVa: A thermodynamic, structural and kinetic study.
Kaplan E, Guichou JF, Chaloin L, Kunzelmann S, Leban N, Serpersu EH, Lionne C
Biochim Biophys Acta 1860 802-13 (2016)
PMID: 26802312

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 37.6 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O68183 (Residues: 1-301; Coverage: 100%)
Gene name: aph(2'')-Id
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 4XR 1 x 4XR
Ligand SIS 1 x SIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 74.22Å b: 65.04Å c: 78.78Å
α: 90° β: 91.18° γ: 90°
R-values:
R R work R free
0.23 0.227 0.278
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Comprehensive review of chemical strategies for the preparation of new aminoglycosides and their biological activities.
Thamban Chandrika et al. (2018)
2 other articles

2 mentions without citation

Overcoming Aminoglycoside Enzymatic Resistance: Design of Novel Antibiotics and Inhibitors.
Zárate et al. (2018)
1 more