› 5c77

X-ray diffraction
2.5Å resolution

A novel protein arginine methyltransferase

Released:
DOI: 10.2210/pdb5c77/pdb
PDB entry 5c77 coloured by chain, front view.
PDB entry 5c77 coloured by chain, side view.
PDB entry 5c77 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for Sfm1 functioning as a protein arginine methyltransferase.
Lv F, Zhang T, Zhou Z, Gao S, Wong CC, Zhou JQ, Ding J
Cell Discov 1 15037 (2015)
PMID: 27462434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein arginine N-methyltransferase SFM1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12314 (Residues: 1-213; Coverage: 100%)
Gene names: OR26.11, SFM1, YOR021C
Sequence domains: Protein arginine N-methyltransferase SFM1-like

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 43.375Å b: 95.62Å c: 58.407Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.214 0.211 0.267
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Mechanistic features of the atypical tRNA m1G9 SPOUT methyltransferase, Trm10.
Krishnamohan et al. (2017)
9 more

2 mentions without citation

Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases.
Strassler et al. (2022)
1 more