Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
E3 ubiquitin-protein ligase NEDD4 and Ubiquitin (preferred)
PDBe Complex ID:
PDB-CPX-143352 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4
Molecule details ›
Chains: A, B
Length: 382 amino acids
Theoretical weight: 45.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: HECT-domain (ubiquitin-transferase)
Length: 382 amino acids
Theoretical weight: 45.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P46934 (Residues: 939-1319; Coverage: 29%)
Sequence domains: HECT-domain (ubiquitin-transferase)
Ubiquitin
Molecule details ›
Chains: C, D
Length: 92 amino acids
Theoretical weight: 10.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Ubiquitin family
Length: 92 amino acids
Theoretical weight: 10.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P0CG48 (Residues: 609-682; Coverage: 11%)
Sequence domains: Ubiquitin family
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 19-ID
Spacegroup:
P32
Expression system: Escherichia coli BL21(DE3)
