› 5c7k

X-ray diffraction
4.6Å resolution

Crystal structure BG505 SOSIP gp140 HIV-1 Env trimer bound to broadly neutralizing antibodies PGT128 and 8ANC195

Released:
DOI: 10.2210/pdb5c7k/pdb
Model geometry
Fit model/data
PDB entry 5c7k coloured by chain, front view.
PDB entry 5c7k coloured by chain, side view.
PDB entry 5c7k coloured by chain, top view.
Source organisms:
Primary publication:
Complete epitopes for vaccine design derived from a crystal structure of the broadly neutralizing antibodies PGT128 and 8ANC195 in complex with an HIV-1 Env trimer.
Kong L, Torrents de la Peña A, Deller MC, Garces F, Sliepen K, Hua Y, Stanfield RL, Sanders RW, Wilson IA
Acta Crystallogr D Biol Crystallogr 71 2099-108 (2015)
PMID: 26457433

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-229770 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (14 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.58 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: S6B291 (Residues: 20-52, 53-76, 77-117, 118-120, 121-123, 124-240; Coverage: 49%)
Sequence domains:
Antibody Fab PGT128 light chain Chain: B
Molecule details ›
Chain: B
Length: 211 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Envelope glycoprotein gp160 Chain: C
Transmembrane protein gp41 Chain: D
Immunoglobulin heavy constant gamma 1 Chain: E
Immunoglobulin kappa constant Chain: F

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 6 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I23
Unit cell:
a: 261.135Å b: 261.135Å c: 261.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.289 0.295
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Native-like Env trimers as a platform for HIV-1 vaccine design.
Sanders et al. (2017)
6 more

14 mentions without citation

The HIV-1 envelope glycoprotein structure: nailing down a moving target.
Ward et al. (2017)
13 more