› 5c8a

X-ray diffraction
2.15Å resolution

Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)

Released:
DOI: 10.2210/pdb5c8a/pdb
PDB entry 5c8a coloured by chain, front view.
PDB entry 5c8a coloured by chain, side view.
PDB entry 5c8a coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural basis for gene regulation by a B12-dependent photoreceptor.
Jost M, Fernández-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elías-Arnanz M, Drennan CL
Nature 526 536-41 (2015)
PMID: 26416754

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable transcriptional regulator Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 206 amino acids
Theoretical weight: 22.12 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 80-285; Coverage: 72%)
Gene name: TT_P0056
Sequence domains: B12 binding domain

Ligands and Environments

3 bound ligands:
Ligand B12 4 x B12
Ligand 5AD 4 x 5AD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 51.43Å b: 99.68Å c: 143.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
20 more

8 mentions without citation

Structural basis for gene regulation by a B12-dependent photoreceptor.
Jost et al. (2015)
7 more