› 5c8j

X-ray diffraction
3.5Å resolution

A YidC-like protein in the archaeal plasma membrane

Released:
DOI: 10.2210/pdb5c8j/pdb
PDB entry 5c8j coloured by chain, front view.
PDB entry 5c8j coloured by chain, side view.
PDB entry 5c8j coloured by chain, top view.
Source organisms:
Primary publication:
A YidC-like Protein in the Archaeal Plasma Membrane.
Borowska MT, Dominik PK, Anghel SA, Kossiakoff AA, Keenan RJ
Structure 23 1715-1724 (2015)
PMID: 26256539

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212284 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody fragment, heavy chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 239 amino acids
Theoretical weight: 25.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Antibody fragment, light chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Protein MJ0480 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 207 amino acids
Theoretical weight: 24.16 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57904 (Residues: 1-198; Coverage: 100%)
Gene name: MJ0480
Sequence domains: Integral membrane protein EMC3/TMCO1-like

Ligands and Environments

1 bound ligand:
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 180.25Å b: 251.42Å c: 96.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.253 0.302
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Nanodiscs in Membrane Biochemistry and Biophysics.
Denisov et al. (2017)
10 more

3 mentions without citation

A YidC-like Protein in the Archaeal Plasma Membrane.
Borowska et al. (2015)
2 more