› 5c8z

X-ray diffraction
1.6Å resolution

ZHD-ZGR complex after ZHD crystal soaking in ZEN for 30min

Released:
DOI: 10.2210/pdb5c8z/pdb
PDB entry 5c8z coloured by chain, front view.
PDB entry 5c8z coloured by chain, side view.
PDB entry 5c8z coloured by chain, top view.
Source organism: Clonostachys rosea
Primary publication:
The structure of a complex of the lactonohydrolase zearalenone hydrolase with the hydrolysis product of zearalenone at 1.60 Å resolution.
Qi Q, Yang WJ, Zhou HJ, Ming DM, Sun KL, Xu TY, Hu XJ, Lv H
Acta Crystallogr F Struct Biol Commun 73 376-381 (2017)
PMID: 28695844

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 31.02 KDa
Source organism: Clonostachys rosea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NKB0 (Residues: 1-264; Coverage: 100%)
Gene name: zhd101
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand ZGR 2 x ZGR
Ligand GOL 4 x GOL
Ligand FMT 3 x FMT
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 74.9Å b: 89.59Å c: 113.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.154 0.168
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Detoxification Strategies for Zearalenone Using Microorganisms: A Review.
Wang et al. (2019)
1 more

3 mentions without citation

Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.
Lyagin et al. (2019)
2 more