› 5cbk

X-ray diffraction
2.46Å resolution

Crystal structure of the strigolactone receptor ShHTL5 from Striga hermonthica

Released:
DOI: 10.2210/pdb5cbk/pdb
PDB entry 5cbk coloured by chain, front view.
PDB entry 5cbk coloured by chain, side view.
PDB entry 5cbk coloured by chain, top view.
Source organism: Striga hermonthica
Primary publication:
Structure-function analysis identifies highly sensitive strigolactone receptors in Striga.
Toh S, Holbrook-Smith D, Stogios PJ, Onopriyenko O, Lumba S, Tsuchiya Y, Savchenko A, McCourt P
Science 350 203-7 (2015)
PMID: 26450211

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 29.99 KDa
Source organism: Striga hermonthica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M5I297 (Residues: 1-271; Coverage: 100%)
Gene name: ShHTL5
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand PE3 2 x PE3
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 102.282Å b: 102.282Å c: 101.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.168 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
34 more

7 mentions without citation

Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.
Xu et al. (2018)
6 more