› 5ce3

X-ray diffraction
2.93Å resolution

The Yersinia YopO - actin complex with MgADP

Released:
DOI: 10.2210/pdb5ce3/pdb
PDB entry 5ce3 coloured by chain, front view.
PDB entry 5ce3 coloured by chain, side view.
PDB entry 5ce3 coloured by chain, top view.
Source organisms:
Entry authors: Lee WL, Singaravelu P, Grimes JM, Robinson RC

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124319 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin Chains: A, C
Molecule details ›
Chains: A, C
Length: 376 amino acids
Theoretical weight: 41.76 KDa
Source organism: Spodoptera frugiperda
UniProt:
  • Canonical: G3CKA6 (Residues: 1-376; Coverage: 100%)
Sequence domains: Actin
Structure domains:
Protein kinase domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 643 amino acids
Theoretical weight: 71.78 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93KQ6 (Residues: 89-729; Coverage: 88%)
Gene names: ERS008652_04012, yopO, ypkA
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 2 x ATP
Ligand CA 2 x CA
Ligand ADP 2 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 108.73Å b: 121.75Å c: 118.58Å
α: 90° β: 104.88° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression system: Escherichia coli

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