› 5ce5

X-ray diffraction
2Å resolution

Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5

Released:
DOI: 10.2210/pdb5ce5/pdb
PDB entry 5ce5 coloured by chain, front view.
PDB entry 5ce5 coloured by chain, side view.
PDB entry 5ce5 coloured by chain, top view.
Source organism: Geobacillus thermocatenulatus
Primary publication:
Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5.
Timucin E, Cousido-Siah A, Mitschler A, Podjarny A, Sezerman OU
Proteins 84 129-42 (2016)
PMID: 26573720

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 43.05 KDa
Source organism: Geobacillus thermocatenulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59260 (Residues: 31-417; Coverage: 93%)

Ligands and Environments

5 bound ligands:
Ligand CA 1 x CA
Ligand ZN 1 x ZN
Ligand P15 1 x P15
Ligand 1PE 1 x 1PE
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I222
Unit cell:
a: 73.305Å b: 128.18Å c: 127.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.204
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Modeling and dynamical analysis of the full-length structure of factor XII with zinc.
Kılınç et al. (2022)

1 mention without citation

Crystal structure of pathogenic Staphylococcus aureus lipase complex with the anti-obesity drug orlistat.
Kitadokoro et al. (2020)