› 5cgv

X-ray diffraction
2.17Å resolution

2009 H1N1 PA endonuclease in complex with L-742,001

Released:
DOI: 10.2210/pdb5cgv/pdb
PDB entry 5cgv coloured by chain, front view.
PDB entry 5cgv coloured by chain, side view.
PDB entry 5cgv coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Identification and characterization of influenza variants resistant to a viral endonuclease inhibitor.
Song MS, Kumar G, Shadrick WR, Zhou W, Jeevan T, Li Z, Slavish PJ, Fabrizio TP, Yoon SW, Webb TR, Webby RJ, White SW
Proc Natl Acad Sci U S A 113 3669-74 (2016)
PMID: 26976575

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.97 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-50, 73-196; Coverage: 24%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand 0N8 1 x 0N8
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P6422
Unit cell:
a: 73.899Å b: 73.899Å c: 127.457Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Structural insights into RNA synthesis by the influenza virus transcription-replication machine.
Pflug et al. (2017)
7 more

5 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
4 more