› 5ci8

X-ray diffraction
2.33Å resolution

Crystal structure of human Tob in complex with inhibitor fragment 1

Released:
DOI: 10.2210/pdb5ci8/pdb
PDB entry 5ci8 coloured by chain, front view.
PDB entry 5ci8 coloured by chain, side view.
PDB entry 5ci8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for inhibition of the Tob-CNOT7 interaction by a fragment screening approach.
Bai Y, Tashiro S, Nagatoishi S, Suzuki T, Yan D, Liu R, Tsumoto K, Bartlam M, Yamamoto T
Protein Cell 6 924-8 (2015)
PMID: 26518565

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156241 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein Tob1 Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 38.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50616 (Residues: 1-345; Coverage: 100%)
Gene names: TOB, TOB1, TROB1
Sequence domains: BTG family
Structure domains: Anti-proliferative protein, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 53Y 1 x 53Y
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 64.975Å b: 64.975Å c: 163.677Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.232 0.285
Expression system: Escherichia coli BL21

Quick links

Citations

1 mention without citation

Homozygous Mutations in BTG4 Cause Zygotic Cleavage Failure and Female Infertility.
Zheng et al. (2020)