› 5cj1

X-ray diffraction
2.1Å resolution

Crystal structure of the coiled coil of MYH7 residues 1526 to 1571 fused to Gp7

Released:
DOI: 10.2210/pdb5cj1/pdb
PDB entry 5cj1 coloured by chain, front view.
PDB entry 5cj1 coloured by chain, side view.
PDB entry 5cj1 coloured by chain, top view.
Source organisms:
Primary publication:
A composite approach towards a complete model of the myosin rod.
Korkmaz EN, Taylor KC, Andreas MP, Ajay G, Heinze NT, Cui Q, Rayment I
Proteins 84 172-189 (2016)
PMID: 26573747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid assembly scaffolding protein; Myosin-7 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 101 amino acids
Theoretical weight: 11.55 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13848 (Residues: 2-53; Coverage: 53%)
  • Canonical: P12883 (Residues: 1527-1571; Coverage: 2%)
Gene names: 7, MYH7, MYHCB
Sequence domains: Phi29 scaffolding protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 56.085Å b: 64.466Å c: 93.594Å
α: 70.53° β: 77.51° γ: 74.15°
R-values:
R R work R free
0.21 0.208 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Molecular Interactions Driving Intermediate Filament Assembly.
Vermeire et al. (2021)
11 other articles

3 mentions without citation

In-Cell Labeling and Mass Spectrometry for Systems-Level Structural Biology.
Chavez et al. (2022)
2 more