› 5cjs

X-ray diffraction
4.3Å resolution

Crystal structure of a monomeric influenza hemagglutinin stem in complex with an broadly neutralizing antibody CR9114

Released:
DOI: 10.2210/pdb5cjs/pdb
Model geometry
Fit model/data
PDB entry 5cjs coloured by chain, front view.
PDB entry 5cjs coloured by chain, side view.
PDB entry 5cjs coloured by chain, top view.
Source organisms:
Primary publication:
A stable trimeric influenza hemagglutinin stem as a broadly protective immunogen.
Impagliazzo A, Milder F, Kuipers H, Wagner MV, Zhu X, Hoffman RM, van Meersbergen R, Huizingh J, Wanningen P, Verspuij J, de Man M, Ding Z, Apetri A, Kükrer B, Sneekes-Vriese E, Tomkiewicz D, Laursen NS, Lee PS, Zakrzewska A, Dekking L, Tolboom J, Tettero L, van Meerten S, Yu W, Koudstaal W, Goudsmit J, Ward AB, Meijberg W, Wilson IA, Radošević K
Science 349 1301-6 (2015)
PMID: 26303961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-211681 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
CR9114 light chain Chains: E, L
Molecule details ›
Chains: E, L
Length: 215 amino acids
Theoretical weight: 22.71 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
CR9114 heavy chain Chains: F, H
Molecule details ›
Chains: F, H
Length: 230 amino acids
Theoretical weight: 24.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Designed influenza hemagglutinin stem #4454, HA1 Chains: C, J
Designed influenza hemagglutinin stem #4454, HA2 Chains: D, K

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 110.869Å b: 110.869Å c: 359.161Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.262 0.303
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

110 review citations

The human antibody response to influenza A virus infection and vaccination.
Krammer F. (2019)
109 more

3 mentions without citation

Stabilisation of Viral Membrane Fusion Proteins in Prefusion Conformation by Structure-Based Design for Structure Determination and Vaccine Development.
Ebel et al. (2022)
2 more