› 5clv

X-ray diffraction
2.5Å resolution

Crystal Structure of KorA-operator DNA complex (KorA-OA)

Released:
DOI: 10.2210/pdb5clv/pdb
PDB entry 5clv coloured by chain, front view.
PDB entry 5clv coloured by chain, side view.
PDB entry 5clv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.
Rajasekar KV, Lovering AL, Dancea F, Scott DJ, Harris SA, Bingle LE, Roessle M, Thomas CM, Hyde EI, White SA
Nucleic Acids Res 44 4947-56 (2016)
PMID: 27016739

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118501 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
TrfB transcriptional repressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 2-97; Coverage: 95%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor
TrfB transcriptional repressor protein Chains: E, F, I, J, M, N
Molecule details ›
Chains: E, F, I, J, M, N
Length: 65 amino acids
Theoretical weight: 7.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 2-66; Coverage: 64%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor
5'-D(CP*CP*AP*AP*GP*TP*TP*TP*AP*GP*CP*TP*AP*AP*AP*CP*TP*TP*GP*GP*)-3' Chains: C, D, G, H, K, L, O, P
Molecule details ›
Chains: C, D, G, H, K, L, O, P
Length: 20 nucleotides
Theoretical weight: 6.13 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 80.46Å b: 114.03Å c: 82.07Å
α: 90° β: 99.59° γ: 90°
R-values:
R R work R free
0.28 0.279 0.292
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Unique Properties of the Alpha-Helical DNA-Binding Protein KfrA Encoded by the IncU Incompatibility Group Plasmid RA3 and Its Host-Dependent Role in Plasmid Maintenance.
Lewicka et al. (2021)
2 more

1 mention without citation

Flexibility of KorA, a plasmid-encoded, global transcription regulator, in the presence and the absence of its operator.
Rajasekar et al. (2016)