› 5com

X-ray diffraction
1.85Å resolution

Crystal structure of Uncharacterized Protein Q187F5 from Clostridium difficile 630

Released:
DOI: 10.2210/pdb5com/pdb
PDB entry 5com coloured by chain, front view.
PDB entry 5com coloured by chain, side view.
PDB entry 5com coloured by chain, top view.
Source organism: Clostridioides difficile 630
Primary publication:
Structures of the DfsB Protein Family Suggest a Cationic, Helical Sibling Lethal Factor Peptide.
Taylor JD, Taylor G, Hare SA, Matthews SJ
J Mol Biol 428 554-560 (2016)
PMID: 26804569

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conjugative transposon protein Tn1549-like, CTn5-Orf2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 22.14 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q187F5 (Residues: 2-172; Coverage: 99%)
Gene name: CD630_18460
Sequence domains: Protein of unknown function (DUF1706)
Structure domains: dinb family like domain

Ligands and Environments

2 bound ligands:
Ligand TAR 1 x TAR
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 127.05Å b: 50.3Å c: 91.72Å
α: 90° β: 118.7° γ: 90°
R-values:
R R work R free
0.172 0.171 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Harnessing the Potential of Killers and Altruists within the Microbial Community: A Possible Alternative to Antibiotic Therapy?
Ikryannikova et al. (2019)
2 other articles

1 mention without citation

Structures of the DfsB Protein Family Suggest a Cationic, Helical Sibling Lethal Factor Peptide.
Taylor et al. (2016)