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› 5cps

X-ray diffraction
1.8Å resolution

Disproportionating enzyme 1 from Arabidopsis - maltotriose soak

Released:
DOI: 10.2210/pdb5cps/pdb
Model geometry
Fit model/data
PDB entry 5cps coloured by chain, front view.
PDB entry 5cps coloured by chain, side view.
PDB entry 5cps coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Dissection of the Maltodextrin Disproportionation Cycle of the Arabidopsis Plastidial Disproportionating Enzyme 1 (DPE1).
O'Neill EC, Stevenson CE, Tantanarat K, Latousakis D, Donaldson MI, Rejzek M, Nepogodiev SA, Limpaseni T, Field RA, Lawson DM
OpenAccess logo J Biol Chem 290 29834-53 (2015)
PMID: 26504082

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in anacceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 564 amino acids
Theoretical weight: 63.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9LV91 (Residues: 46-576; Coverage: 92%)
Gene names: At5g64860, DPE1, MXK3.9
Sequence domains: 4-alpha-glucanotransferase
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand EDO 16 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 69.41Å b: 73.13Å c: 79.12Å
α: 65.46° β: 69.48° γ: 67.09°
R-values:
R R work R free
0.154 0.153 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Transitory Starch Metabolism in Guard Cells: Unique Features for a Unique Function.
Santelia et al. (2017)
4 more

3 mentions without citation

Structural Dissection of the Maltodextrin Disproportionation Cycle of the Arabidopsis Plastidial Disproportionating Enzyme 1 (DPE1).
O'Neill et al. (2015)
2 more