› 5cqq

X-ray diffraction
3.1Å resolution

Crystal structure of the Drosophila Zeste DNA binding domain in complex with DNA

Released:
DOI: 10.2210/pdb5cqq/pdb
PDB entry 5cqq coloured by chain, front view.
PDB entry 5cqq coloured by chain, side view.
PDB entry 5cqq coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of Zeste-DNA Complex Reveals a New Modality of DNA Recognition by Homeodomain-Like Proteins.
Gao GN, Wang M, Yang N, Huang Y, Xu RM
J Mol Biol 427 3824-33 (2015)
PMID: 26478222

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118512 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Regulatory protein zeste Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 10.1 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09956 (Residues: 51-130; Coverage: 14%)
Gene names: CG7803, z
Sequence domains: Myb/SANT-like DNA-binding domain
DNA (5'-D(*CP*TP*GP*TP*TP*TP*TP*CP*CP*AP*CP*TP*CP*GP*TP*TP*TP*TP*T)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 19 nucleotides
Theoretical weight: 5.72 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*AP*AP*CP*GP*AP*GP*TP*GP*GP*AP*AP*AP*AP*CP*AP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 19 nucleotides
Theoretical weight: 5.93 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 51.007Å b: 59.705Å c: 202.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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