› 5cr6

X-ray diffraction
1.98Å resolution

Structure of pneumolysin at 1.98 A resolution

Released:
DOI: 10.2210/pdb5cr6/pdb
PDB entry 5cr6 coloured by chain, front view.
PDB entry 5cr6 coloured by chain, side view.
PDB entry 5cr6 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
The Crystal Structure of Pneumolysin at 2.0 Å Resolution Reveals the Molecular Packing of the Pre-pore Complex.
Marshall JE, Faraj BH, Gingras AR, Lonnen R, Sheikh MA, El-Mezgueldi M, Moody PC, Andrew PW, Wallis R
Sci Rep 5 13293 (2015)
PMID: 26333773

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pneumolysin Chain: D
Molecule details ›
Chain: D
Length: 471 amino acids
Theoretical weight: 52.93 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04IN8 (Residues: 1-471; Coverage: 100%)
Gene names: SPD_1726, ply
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 27.006Å b: 127.71Å c: 172.285Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.248 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Streptococcus pneumoniae's Virulence and Host Immunity: Aging, Diagnostics, and Prevention.
Brooks et al. (2018)
6 more

12 mentions without citation

Cholesterol-dependent cytolysins: from water-soluble state to membrane pore.
Christie et al. (2018)
11 more