› 5crd

X-ray diffraction
2.08Å resolution

Wild-type human skeletal calsequestrin

Released:
DOI: 10.2210/pdb5crd/pdb
PDB entry 5crd coloured by chain, front view.
PDB entry 5crd coloured by chain, side view.
PDB entry 5crd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization of Two Human Skeletal Calsequestrin Mutants Implicated in Malignant Hyperthermia and Vacuolar Aggregate Myopathy.
Lewis KM, Ronish LA, Ríos E, Kang C
J Biol Chem 290 28665-74 (2015)
PMID: 26416891

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calsequestrin-1 Chain: A
Molecule details ›
Chain: A
Length: 362 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31415 (Residues: 35-396; Coverage: 100%)
Gene names: CASQ, CASQ1
Sequence domains: Calsequestrin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 59.17Å b: 145.132Å c: 110.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.217
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Core skeletal muscle ryanodine receptor calcium release complex.
Dulhunty et al. (2017)
6 more

2 mentions without citation

Functions and mechanisms of protein disulfide isomerase family in cancer emergence.
Rahman et al. (2022)
1 more