› 5cwa

X-ray diffraction
2.1Å resolution

Structure of Anthranilate Synthase Component I (TrpE) from Mycobacterium tuberculosis with inhibitor bound

Released:
DOI: 10.2210/pdb5cwa/pdb
PDB entry 5cwa coloured by chain, front view.
PDB entry 5cwa coloured by chain, side view.
PDB entry 5cwa coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Structure and inhibition of subunit I of the anthranilate synthase complex of Mycobacterium tuberculosis and expression of the active complex.
Bashiri G, Johnston JM, Evans GL, Bulloch EM, Goldstone DC, Jirgis EN, Kleinboelting S, Castell A, Ramsay RJ, Manos-Turvey A, Payne RJ, Lott JS, Baker EN
Acta Crystallogr D Biol Crystallogr 71 2297-308 (2015)
PMID: 26527146

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161437 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate synthase component 1 Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 55.5 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: P9WFX2 (Residues: 1-511; Coverage: 99%)
Gene names: MT1644, trpE
Sequence domains:
Structure domains: Anthranilate synthase

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
Ligand 0GA 1 x 0GA
Ligand UNL 1 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P6422
Unit cell:
a: 156.406Å b: 156.406Å c: 128.384Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.253
Expression system: Mycolicibacterium smegmatis

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Citations

5 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
4 more

4 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
3 more