› 5cwz

X-ray diffraction
2.9Å resolution

Crystal structure of the kinase domain of human TRAF2 and NCK-interacting protein kinase

Released:
DOI: 10.2210/pdb5cwz/pdb
PDB entry 5cwz coloured by chain, front view.
PDB entry 5cwz coloured by chain, side view.
PDB entry 5cwz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TNIK inhibition abrogates colorectal cancer stemness.
Masuda M, Uno Y, Ohbayashi N, Ohata H, Mimata A, Kukimoto-Niino M, Moriyama H, Kashimoto S, Inoue T, Goto N, Okamoto K, Shirouzu M, Sawa M, Yamada T
Nat Commun 7 12586 (2016)
PMID: 27562646

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193843 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAF2 and NCK-interacting protein kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 308 amino acids
Theoretical weight: 35.08 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9UKE5 (Residues: 11-314; Coverage: 22%)
Gene names: KIAA0551, TNIK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Unit cell:
a: 50.215Å b: 123.896Å c: 158.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.281
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

20 review citations

Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications.
Ishiguro et al. (2017)
19 more

2 mentions without citation

Structural Insight into TNIK Inhibition.
Kukimoto-Niino et al. (2022)
1 more