› 5cxr

X-ray diffraction
2Å resolution

Influenza endonuclease complexed with 4-bromopyrazole

Released:
DOI: 10.2210/pdb5cxr/pdb
PDB entry 5cxr coloured by chain, front view.
PDB entry 5cxr coloured by chain, side view.
PDB entry 5cxr coloured by chain, top view.
Source organism: Influenza A virus (A/California/02/2010(H1N1))
Primary publication:
Rapid experimental SAD phasing and hot-spot identification with halogenated fragments.
Bauman JD, Harrison JJ, Arnold E
IUCrJ 3 51-60 (2016)
PMID: 26870381

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 24.56 KDa
Source organism: Influenza A virus (A/California/02/2010(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: M9QLS3 (Residues: 1-204; Coverage: 29%)
Gene name: PA

Ligands and Environments

4 bound ligands:
Ligand MN 3 x MN
Ligand EDO 1 x EDO
Ligand BYZ 5 x BYZ
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 87.905Å b: 102.451Å c: 66.062Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Twenty years on: the impact of fragments on drug discovery.
Erlanson et al. (2016)
1 more

1 mention without citation

Rapid experimental SAD phasing and hot-spot identification with halogenated fragments.
Bauman et al. (2016)