› 5czh

X-ray diffraction
2.8Å resolution

EGFR L858R MUTANT IN COMPLEX WITH AN OPTIMAL PEPTIDE SUBSTRATE

Released:
DOI: 10.2210/pdb5czh/pdb
PDB entry 5czh coloured by chain, front view.
PDB entry 5czh coloured by chain, side view.
PDB entry 5czh coloured by chain, top view.
Source organisms:
Primary publication:
EGF-receptor specificity for phosphotyrosine-primed substrates provides signal integration with Src.
Begley MJ, Yun CH, Gewinner CA, Asara JM, Johnson JL, Coyle AJ, Eck MJ, Apostolou I, Cantley LC
Nat Struct Mol Biol 22 983-90 (2015)
PMID: 26551075

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132680 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.68 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 694-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Peptide substrate Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.25 KDa
Source organism: Escherichia coli H489
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I23
Unit cell:
a: 144.566Å b: 144.566Å c: 144.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.22
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

5 review citations

Homing in: Mechanisms of Substrate Targeting by Protein Kinases.
Miller et al. (2018)
4 more

7 mentions without citation

The Src module: an ancient scaffold in the evolution of cytoplasmic tyrosine kinases.
Shah et al. (2018)
6 more